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Histology Procedure Manuals |
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| The manuals for this section have been compiled by Susan L. Wall, HT(ASCP). These manuals are in Adobe Acrobat PDF format and require the Acrobat Reader for vi... |
/ United States
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E.M. PROCESSING SCHEDULE - EPOXY RESIN |
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| Fix tissue in 2.5% glutaraldehyde in 0.1M sodium cacodylate buffer at 4oC, for a minimum of 4 hours. Tissue should be cut into approx. 1mm cubes for fixing. Th... |
/ United Kingdom
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EMS mutagenesis |
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| EMS is poweful mutagen and a suspected carcinogen. Wear gloves and work in fume hood. Use disposable plasticware and inactivate mutagen before disposal as outl... |
/ United States
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Science Lab Techniques |
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| The surface of a liquid confined in a cylinder curves to form what is known as a meniscus. The meniscus of most liquids curves up the sides of the container, m... |
/ United States
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Quick DNA Plasmid Prep. |
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| This is a very fast mini-prep protocol which is suitable for sequence analysis and restriction digests. Although the yield is higher than Protocol D.1, there i... |
Boston United States
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Southern Blotting |
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| Electrophoresis of DNA is carried out in a neutral agarose gel system. Prepare a 0.8-1% agarose gel containing 1x TAE buffer. Ethidium bromide can be added to ... |
/ United States
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Conjugation of monoclonal antibodies |
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| In this series of web pages, protocols, notes, and various illustrations are given to aid in the conjugation of proteins--principally monoclonal antibodies--to... |
/ United States
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